Contact

jose.ordovas-montanes[at]childrens.harvard.edu

Lab Home

300 Longwood Ave. 

Boston, MA 02115

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WHAT WE DO

APPROACH

Integrating fundamental immunological insights with innovative technologies, experimentation, and computational approaches

HUMAN IMMUNOLOGY

Understanding human inflammatory diseases at single-cell resolution in barrier tissues

TISSUE STEM CELLS

Defining the mechanisms of inflammatory adaptation and memory by epithelial stem cells

TISSUE BIOLOGY

Building a framework for how inflammation changes the states of  epithelial, stromal, and immune cells in tissues, and  reshapes their interactions

APPROACH

We are developing a training environment where we apply emerging techniques to answer fundamental questions of biological and clinical relevance in barrier tissue biology.

 

Conceptually, for a barrier tissue to effectively learn from previous immunological experiences, it must sense, adapt, and store this information (i.e. memory) in readily accessible permanent resident cell types. We are interested in exploring how memories of previous immune events (i.e. inflammatory memory) enables barrier tissues (airway, intestine, and skin) to recall diverse environmental exposures, informing future responses. We are particularly interested in further understanding our discovery that epithelial stem cells, amongst other parenchymal, stromal, neuronal, and immune cell subsets, can form inflammatory memory, raising the possibility of distributed memory formation.

 

Technically, we utilize single-cell RNA-sequencing (scRNA-seq), organoid models, epigenetic profiling, flow cytometry, and microscopy applied to human health and disease. We then build testable models to explore with humans (organoids, treatment response vs. failure) or with mice (genetics, optogenetics, cellular immunology), towards the aim of improving disease understanding and treatment. 

 

HUMAN IMMUNOLOGY

We begin by generating maps of tissues using single-cell RNA-sequencing and epigenomic approaches to understand the cell types, subsets, and states which constitute a tissue. We are particularly motivated to apply these techniques to human inflammatory diseases at barrier tissues. Ongoing work focuses on allergic inflammation in the upper airway (chronic rhinosinusitis), as well as inflammatory bowel diseases in the intestine (Crohn's disease and ulcerative colitis). By studying these maps, we are generating new insights into the cellular basis of inflammatory diseases, and building new in vitro and in vivo models to experimentally test our findings.  

 

TISSUE STEM CELLS

Enabled by directed inquiries into human epithelial cell types, subsets, and states, our work has suggested that cell types beyond tissue-resident lymphocytes may adapt and harbor memories of inflammation. In light of these findings, we are exploring how epithelial stem cells in barrier tissues integrate inflammatory cues, and contribute to the persistence of human diseases. Our first-in-human discovery of inflammation dictating epithelial stem cell fates has fundamental implications for our conceptual and clinical understanding of inflammatory diseases. We are leveraging stem cell and organoid models to explore the molecular basis of our findings, including intersections with classical developmental biology. 

 

TISSUE BIOLOGY

Our research to date has made several pioneering discoveries in the inter-cellular interactions of immune cells in their relevant tissue context. Our overarching scientific goals are to integrate the knowledge we and others are generating towards elucidating the principles of how inflammation shapes barrier tissues. Working towards this will aid in realizing a framework for inflammatory adaptation by mammalian tissues, with direct implications for chronic human diseases, an area in need of therapies beyond those targeting traditional immune mechanisms. Fundamentally, we are interested in programming and re-programming memories in barrier tissues.  

 

COLLABORATORS

Brigham and Women's Allergy and ImmunologyTogether with Dan Dwyer, Katie Buchheit, Tanya Laidlaw, Josh Boyce, and Nora Barrett we are exploring how allergic inflammation shapes the respiratory epithelium. 

Leslie Kean Lab (now at Boston Children's): Together with Leslie and Betty Zheng, we are charting the cellular basis of pediatric inflammatory bowel disease, including both Crohn's disease and ulcerative colitis. 

Musa Mhlanga Lab: Together with Musa, we are studying the epigenetic mechanisms of inflammatory memory. 

Seth Rakoff-Nahoum Lab: Together with Seth, we are exploring specific questions of microbe-host interactions in the intestine and respiratory tract, along with broader questions of how inflammation shapes these tissues. 

Alex Shalek Lab: Together with Alex, we are continuing to build single-cell RNA-sequencing approaches for understanding the cellular communities in our tissues.  

Scott Snapper LabTogether with Scott, we are motivated to understand the genetic mechanisms underlying cellular phenotypes in very early onset pediatric inflammatory bowel disease.